Journal: PLOS Genetics
Article Title: Plagl1 regulates the retinal progenitor cell to Müller glial cell transition
doi: 10.1371/journal.pgen.1012020
Figure Lengend Snippet: (A) Establishment of a conditional transgenic approach to activate gene expression in perinatal/postnatal Müller glia using Slc1a3-CreERT driver mice and a Rosa-zsGreen reporter line. (B) 4-OHT treatment of Slc1a3-CreERT;Rosa-zsGreen mice at P5, P7 and P14, followed by analysis of the distribution of zsGreen + cells at P21. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 50µm. (C) Targeting strategy to generate a floxed allele of Plagl1 (from ), and crosses to Slc1a3-CreERT to generate a Müller glia specific Plagl1 -cKO. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/gjcg2ar (D) Tamoxifen injections were administered at P14 every second day and animals were assessed at P21. Sox9 and zsGreen immunostaining in P21 Slc1a3-CreERT ; Rosa-zsGreen control and Slc1a3-CreERT;Plagl1 floxed retinas (i.e., Plagl1 -cKOs). Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 25µm. (E) Total zsGreen + (ZsG + ) and Sox9 + cells were quantified per field. Mean values and error bars representing s.e.m. were plotted. p-values calculated with unpaired t-test.
Article Snippet: Plagl1 fl/+ males (received from Pengpeng Bi) [ ] were crossed with Slc1a3-CreERT females (JAX:012586) to generate Plagl1 cKOs.
Techniques: Transgenic Assay, Gene Expression, Immunostaining, Control